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. 2021 May 20;12(6):517. doi: 10.1038/s41419-021-03782-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A pcDNA3.1-TSP50 was transfected into L02 cell to overexpress TSP50 and efficient expression of TSP50 was examined by Western blot. B, C The L02 cell viability and BrdU absorbance were detected in cells after pcDNA3.1-TSP50 transfection by MTT and BrdU assay. D–K After pcDNA3.1-TSP50 transfected in L02 cells, the ECAR, OCR, glucose consumption, lactate production, LDH activity, ATP levels, G6P levels and 2-PG levels were analyzed. L Effect of 2-DG on TSP50-overexpressed L02 cells, the expression of TSP50 was examined by Western blot. M, N The cell viability and BrdU absorbance were detected by MTT and BrdU assay. O–R glucose consumption, lactate production, LDH activity and ATP levels were analyzed. The aerobic glycolysis values were normalized to protein levels. N = 3 biologically independent replicates. t-Test or one-way ANOVA statistical analysis were used. Data were presented as means ± s.d. *P < 0.05, ** P < 0.01. ns, no significance.