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. 2021 May 20;12(6):517. doi: 10.1038/s41419-021-03782-w

Fig. 5. TSP50-mediated acetylation is essential for maintaining low level of tetrameric PKM2.

Fig. 5

A Comparison of PKM2 oligomer states in different cells. After extracting the cell protein and treating with glutaraldehyde, immunoblotting was performed with anti-PKM2 for oligomer states. B–D PKM2 oligomer states were detected in TSP50-overexpressed L02 cells and TSP50-knockdowned HCC cells. E TSP50 was overexpressed in L02 cells and knocked down in Huh7 cells and Bel7402 cells. Then, cells were harvested and followed by IP with anti-PKM2, Western blot analysis was performed with anti-PKM2, Acetylation, p-Tyr, p-Thr p-Ser and O-Glycosylation. Interaction of PKM2 with KAT9 and SIRT2 were analyzed by co-IP method. F–S After TEPP46 treatment, the cell viability, BrdU absorbance, glucose consumption, lactate production, LDH activity and ATP levels were analyzed. All aerobic glycolysis values were normalized to protein levels. N = 3 biologically independent replicates. t-Test statistical analysis was used. Data were presented as means ± s.d. *P < 0.05, ** P < 0.01.