Fig. 7. TSP50 mediates the cell proliferation and aerobic glycolysis changes partially dependent on PKM2 K433 acetylation.

A The pcDNA3.1-TSP50 and Flag-PKM2 WT, Flag-PKM2 K62R or Flag-PKM2 K433R mutant plasmids were co-transfected into L02, Huh7 and Bel7402 cells, respectively. B–Y The cell viability, BrdU absorbance, glucose consumption, lactate production, ATP levels, G6P levels, 2-PG levels and ECAR levels were detected. The aerobic glycolysis values were normalized to protein levels. N = 3 biologically independent replicates. One-way ANOVA statistical analysis was used. Data were presented as means ± s.d. *P < 0.05, ** P < 0.01. ns, no significance.