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. 2021 May 20;10(5):43. doi: 10.1038/s41389-021-00330-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B Cell models with or without PSMC2 knockdown were constructed by transfecting shPSMC2 or shCtrl. The knockdown efficiency of PSMC2 in GBC-SD and SGC-996 cells was assessed by qPCR (A) and western blotting (B). C MTT assay was employed to show the effects of PSMC2 on cell proliferation of GBC-SD and SGC-996 cells. D Plate clone formation assay was applied to investigate the effects of PSMC2 knockdown on colony formation ability of GBC-SD and SGC-996 cells. E Flow cytometry was performed to detect cell apoptosis of GBC-SD and SGC-996 cells with or without PSMC2 knockdown. F Human apoptosis antibody array was utilized to analyze the regulatory ability of PSMC2 on the expression of apoptosis-related proteins in GBC-SD cells. Data were shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.