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. 2021 May 20;6:75. doi: 10.1038/s41541-021-00334-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Mice (N = 6/group) were immunized with NY-ESO-1 plus various adjuvants on days 0, 14, and 28. The levels of IgG (a), IgG1 and IgG2c (b) specific for NY-ESO-1 were detected by ELISA one week after the third immunization. Bars represent median. c, d The frequencies of IFN-γ-secreting CD4⁺ and CD8⁺ T cells were examined by intracellular IFN-γ staining (N = 3/group). e, f The average number of IL-4/IFN-γ-secreting splenocytes was calculated (N = 3/group). Bars represent means and SEM. *P < 0.05, **P < 0.01, ***P < 0.001.