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. 2021 May 20;6:76. doi: 10.1038/s41541-021-00340-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — As dengue circulates in its natural ecological niche, the virus undergoes selection pressures imposed by the alternating human host and mosquito vector. When the virus is transferred to a new ecological niche in vitro and continually passaged, the past selective pressures of host and vector are replaced with those of the new niche. Images are created using Biorender.