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. 2021 May 20;12:2989. doi: 10.1038/s41467-021-23242-5

Fig. 6. iPGCs migrate to the genital ridge after embryo transplantation.

Fig. 6

a Real-time fluorescence observation of genital ridges isolated from chicken embryos transplanted with iPGCs, PGCs, and CEFs. iPGCs, PGCs, and CEFs were treated with pKH26, showing red fluorescence before injection. Green fluorescence was expressed by the OSNL vectors carrying GFP originally transfected into CEFs. Scale bar: 2 mm. (n = 9 chick embryos form three independent experiments). b Fluorescence observation of frozen embryonic genital ridge sections after iPGC, PGC, and CEF transplantation. Scale bar: 70 μm. (n = 9 chick embryos form three independent experiments).