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. 2021 May 7;12:680068. doi: 10.3389/fimmu.2021.680068

Figure 2.

Figure 2

PKM2 augments TLRs-induced activation of BMDMs. (A–D) BMDMs, infected with lentivirus expressing PKM2 (PKM2-LV) or negative control lentivirus (NC-LV) for 3 days, were stimulated by LPS (100 ng/mL), R848 (1μg/mL) and CpG 1826 (1 μM). FACS analysis of CD86 (A) and CD40 (B) expression at 24 hours. ELISA analysis of the levels of IL-12 and TNF-α secreted by BMDMs at 24 hours (C). Western blot analysis of the phosphorylation levels of p38, Erk, JNK, and p65 at 1 hour (D). (E–H) BMDMs, infected with lentivirus expressing negative control-RNAi (RNAi-NC-LV) or PKM2-specific RNAi (RNAi-PKM2-LV) for 3 days, were stimulated by LPS (100 ng/mL), R848 (1μg/mL) and CpG 1826 (1 μM). FACS analysis of CD86 (E) and CD40 (F) expression at 24 hours. ELISA analysis of the levels of IL-12 and TNF-α secreted by BMDMs at 24 hours (G). Western blot analysis of the phosphorylation levels of p38, Erk, JNK, and p65 at 1 hour (H). The data shown represent the means of three independent experiments and the error bars represent the S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by ANOVA tests; ns denotes p > 0.05.