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. 2021 May 7;12:680068. doi: 10.3389/fimmu.2021.680068

Figure 5.

Figure 5

PKM2 augments the activations of TLR4/TLR7/TLR9 pathways by promoting Pyk2 activation. (A–C) BMDMs were treated with PKM2-IN (2.5, 5, 10 μM) for 2 hours followed by stimulations of LPS (100 ng/mL), R848 (1 μg/mL) and CpG-1826 (1 μM). Western blot analysis of the phosphorylation levels of Pyk2 at 1 hour. (D–F) BMDMs were treated with TAE226 (1, 2, 4 μM) for 2 hours followed by stimulations of LPS (100 ng/mL), R848 (1 μg/mL) and CpG-1826 (1 μM). FACS analysis of CD86 and CD40 expression at 24 hours (D, E). ELISA analysis of the levels of IL-12 and TNF-α secreted by BMDMs at 24 hours (F). (G) Co-IP analysis of the interaction between PKM2 and Pyk2. The data shown represent the means of three independent experiments and the error bars represent the S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by ANOVA tests.