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. 2021 May 21;21:463. doi: 10.1186/s12879-021-06173-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Performance of the emm75 qPCR. qPCR targeting the emm75 or speB gene was performed on genomic DNA isolated from 10-fold dilutions of S. pyogenes M75. The speB qPCR was used to provide a benchmark for the performance of the emm75 qPCR, using FAM-conjugated probes for both reactions. Each point represents the mean and standard deviation (SD) of duplicate reactions