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. 2021 May 7;8:660072. doi: 10.3389/fmolb.2021.660072

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Copyright © 2021 Chang, Guan, Wang, Chen, Zhu, Wei and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complex IV subunit 4 isoform 2 (Cox4i2) depletion attenuated HHV7-induced ferroptosis. (A,B) Protein and relative mRNA levels of FTH, TFRC, SLC7A11, SLC3A2, and GPX4 were determined by Western Blotting and RT-qPCR in vivo. (C,D) Protein and relative mRNA levels of FTH, TFRC, IREB2, SLC7A11, SLC3A2, and GPX4 were determined by Western Blotting and RT-qPCR in HHV7-treated RSC96 cells with or without shCoc4i2. (E) Image of PGSK staining of RSC96 cells. Data are presented as the mean ± standard deviation, n = 3. Statistical significance was assessed with Student’s t-test and one-way ANOVA. **P < 0.01; *P < 0.05.