FIGURE 6.

YchF interacts with the ribosomal proteins uS11 and bS21 (A,B) as described in Figure 5, but the enriched material was analyzed with antibodies against bS21 and uS11. As a control, purified ribosomes as in Figure 5 were also loaded. (C) Positions of ribosomal proteins that were found to cross-link to YchF. The 30 S subunit is displayed in light gray and the 50 S subunit in dark gray (PDB 5 mdz) (James et al., 2016); please note that the ribosomal proteins bS1 and bL7/12 are not visualized in this structure.