FIGURE 8.

YchF reduces leaderless mRNA translation. The gfp-reporter sequence of plasmids pMG991 and pMG999 were cloned into the pCDFDuett vector, generating plasmids pCDF-991 (canonical translation) and pCDF-999 (lmRNA translation). The indicated strains were grown on LB-medium in the presence of 0.002% arabinose and at OD₆₀₀ = 0.4, the expression of the reporter plasmids was induced with 1 mM IPTG and cells were grown at 37°C. At the indicated time points, cells were harvested, washed once in PBS and were then resuspended in PBS. Samples were analyzed as described in the legend in Figure 7. The mean values from at least three experiments are shown and the error bar reflects the SEM.