FIGURE 9.
The absence of YchF increases resistance against MazF-dependent mRNA cleavage. (A) Plasmid-encoded mazF was expressed for one hour when indicated (+) in wild-type and ΔychF cells, grown on M63 minimal medium. Subsequently, 35S-labeled cysteine and methionine were added and samples were taken after 1 and 2 min of labeling and directly TCA-precipitated. Samples were then separated by SDS PAGE and analyzed by phosphor-imaging. A representative image of at least three independent experiments is shown. (B) 108 cells of the cultures before (0) or after induction (1) were precipitated with 10% TCA and the pellet was separated by SDS-PAGE and after western transfer analyzed with antibodies against YchF and His-tagged MazF.