Figure 4.

Determination of the CXCL8 chemokine that might attract DC. A transwell migration assay was performed. Splenocytes from mice were exposed to different concentrations of CXCL8 (0, 10, 25, 50 and 100 ng/mL) for 6 hours before quantifying DCs by flow cytometric analysis. (A, B) Flow cytometry was performed using CD11c and MHC-II gating strategy (A) to quantify DC (B). Significant migration of DCs was observed upon treatment with CXCL8. For each treatment, the populations of DCs in the low- and high-dose groups were compared to the untreated group population.