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. 2021 May 7;12:667177. doi: 10.3389/fimmu.2021.667177

Figure 6.

Figure 6

Antagonism of CXCR2 inhibits DC activation and prevents CD8+ T cell and DC infiltration in the TME. (A–E) CT26 murine colon tumor cells were subcutaneously injected into BALB/c mice. Since the day tumor cells were inoculated, the mice were treated with DMSO (control) and danirixin for 2 weeks. (A) The qRT-PCR analysis showed the statistical significance of three DC activation genes, CD54, CD83, CD86 between the group treated with danirixin and the control group. (B) Flow cytometric analysis of the tumor-infiltrated DC (CD11c+ MHC-II+) proportion in CT26 tumors after treatment with danirixin, (C) Flow cytometric analysis of DCs migration with or without danirixin treatment, (D) flow cytometry analysis of IFN-γ expressing CD8+ T cell and (E) Granzyme B-expressing CD8+ T cells in the TME of tumors generated with CT26 with or without treated with danirixin. Data are shown as the mean ± SEM. Statistical analyses were performed by a pairwise Student t test (n=4 per group). ns: p > 0.05, *p < 0.05, **p < 0.01.