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. 2021 May 7;12:615998. doi: 10.3389/fphar.2021.615998

FIGURE 4.

FIGURE 4

R1 enhances neural reconstruction by stimulating neurogenesis after ischemic stroke. Representative images of the infarction area costained with antibodies against (A) DCX (green, marker of migrating and immature neurons) and EdU (red, marker of proliferating cells) on day 7 after R1 treatment, yellow arrows indicate migrating neuroblasts (EdU+/DCX+ cells) (B) Nestin (green, marker of proliferating NPCs) and EdU (red) on day 7 after R1 treatment, yellow arrows indicate proliferating NPCs (EdU+/Nestin+ cells) (C) NeuN (green, marker of mature neurons) and EdU (red) on day 28 after R1 treatment, yellow arrows indicate newly formed mature neurons (EdU+/NeuN+ cells). DAPI (blue) indicates the nucleus, Scale bar = 50 μm (D-F) Quantitative analysis of (A-C) in the striatum region respectively after R1 treatment. n = 3 brains per group. Data are expressed as the mean ± SD and were analyzed by ANOVA. # p< 0.05, ## p< 0.01 vs. Sham group; * p< 0.05, **p< 0.01 vs. MCAO/R group.