FIGURE 6.

R1 increases neurotrophic factor expression and restores disrupted neural synaptic function after ischemic stroke. The serum and cortex tissue levels of neurotrophic factors, such as BDNF (A, B), NGF (C, D), and NT-4 (E, F) were detected by ELISA. (G) Representative images of the infarction area costained with antibodies against BDNF (red) and NeuN (green) on day 28 after R1 treatment, yellow arrows indicate NeuN-positive neurons expressed BDNF, DAPI (blue) indicates the nucleus, Scale bar = 50 μm. Representative images of immunoblotting (H) and quantification of the relative protein levels of SYN (I), PSD95 (J), MAP-1 (K) and Tau-1 (L) in the infarct cortex region of the Sham, MCAO/R, R1, and MCAO/R + R1 groups on day 28 after MCAO surgery. n = 3 in each group. Data are expressed as the mean ± SD and were analyzed by ANOVA. ^# p< 0.05, ^## p< 0.01 vs. Sham group; ^* p< 0.05, **p< 0.01 vs. MCAO/R group.