Figure 5.

TLR4 silencing prevents microRNA-489-3p inhibitor-induced hyperproliferation in KCs. (A) Transfection efficiency of si-TLR4. (B) Expression level of TLR4 in HaCaT cells transfected with different plasmids was detected via reverse transcription-quantitative PCR. (C) Cell Counting Kit-8 assays were used analyze cell proliferation. (D) Colony forming ability was analyzed using a colony formation assay, (E) and the results were quantified. (F) Flow cytometry was used to assess the cell cycle and (G) the results of G₂/M were quantified. ^*P<0.05 vs. inhibitor-con + si-con; ^#P<0.05 vs. inhibitor + si-con. con, negative control; TLR4, Toll-like receptor 4; si, small interfering RNA.