TABLE 1.
Sample | Raw reads | Clean reads | Q20 (%) | Q30 (%) | GC content (%) | Total mapped reads |
A_1 | 54542030 | 53752304 | 98.71 | 95.68 | 45.04 | 41320416 (76.87%) |
A_2 | 79607262 | 78327428 | 98.68 | 95.59 | 44.67 | 60458266 (77.19%) |
A_3 | 66307584 | 65005808 | 98.69 | 95.6 | 44.67 | 50076660 (77.03%) |
B_1 | 61825070 | 60940514 | 98.55 | 95.17 | 44.91 | 47642222 (78.18%) |
B_2 | 61776038 | 60874120 | 98.71 | 95.66 | 44.81 | 47623800 (78.23%) |
B_3 | 62783660 | 61896096 | 98.72 | 95.65 | 44.75 | 48219096 (77.90%) |
C_1 | 65584430 | 64443166 | 98.74 | 95.73 | 44.65 | 50675552 (78.64%) |
C_2 | 66911796 | 65761656 | 98.67 | 95.52 | 44.68 | 51653690 (78.55%) |
C_3 | 70930988 | 69608456 | 98.58 | 95.29 | 44.2 | 54910004 (78.88%) |
D_1 | 64323328 | 63325728 | 98.66 | 95.46 | 44.22 | 48845080 (77.13%) |
D_2 | 53106480 | 52366662 | 98.73 | 95.72 | 44.51 | 40206360 (76.78%) |
D_3 | 61134368 | 60176382 | 98.66 | 95.47 | 44.35 | 46130222 (76.66%) |
E_1 | 64246102 | 63464652 | 98.69 | 95.53 | 44.45 | 49456492 (77.93%) |
294E_2 | 47298170 | 46481888 | 98.68 | 95.48 | 44.36 | 36320930 (78.14%) |
E_3 | 58093350 | 57090472 | 98.75 | 95.69 | 44.49 | 44561666 (78.05%) |
F_1 | 59865570 | 58791448 | 98.55 | 95.12 | 44.19 | 45797898 (77.90%) |
F_2 | 67186956 | 66240678 | 98.75 | 95.71 | 44.1 | 51789204 (78.18%) |
F_3 | 60845708 | 59572020 | 98.72 | 95.6 | 44.13 | 46554538 (78.15%) |
G_1 | 73520470 | 72003528 | 98.78 | 95.73 | 43.98 | 56418766 (78.36%) |
G_2 | 54733094 | 53300870 | 98.79 | 95.77 | 44.01 | 42180466 (79.14%) |
G_3 | 68004602 | 67124008 | 98.65 | 95.35 | 43.96 | - |
A, 40 DAF; B, 60 DAF; C, 80 DAF; D, 0 DAP; E, 4 DAP; F, 8 DAP; G, 12 DAP. _1, _2, and _3 represent the triplicate analyses of each sample. Q20 is the percentage of bases with a Phred score greater than 20, whereas Q30 is the percentage of bases with a Phred score greater than 30.