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. 2021 May 7;9:656795. doi: 10.3389/fcell.2021.656795

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Copyright © 2021 Tremblay, Mompart, Lopez, Quaranta, Bergoglio, Hashim, Bonnet, Alric, Mas, Trouche, Vignard, Ferrand, Mirey and Fernandez-Vidal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CDT causes a slowdown of DNA replication in the late S phase. Cell cycle analysis by flow cytometry of HeLa (A,B) and U2OS (C,D) cells treated or not with 2.5 ng/ml of wild-type or catalytically inactive CDT mutant (Mut) from E. coli (CDT Ec) (A,C) or H. ducreyi (CDT Hd) (B,D) for 16 h was performed. (A,C) Left panel: representative flow cytometry of cells treated with CDT Ec is shown. EdU incorporation is plotted against the cellular DNA content (DAPI). Quantification of G1, G2, and late S cell population with low EdU (red boxes) is indicated. Right panel: data represent the percentage of EdU weakly positive cells in the late S phase. At least 10,000 events were analyzed per sample using the CytExpert software (mean ± SEM of at least three independent experiments) (^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ns, not significant).