Flow cytometric analysis of CD4+ peripheral blood populations over time. Quantification of frequencies for the following dependent variables: (a) CD4+ lymphocytes, (b) CD4+CD127lowCD25+ Tregs, (c) CD4+CD127highCD25+ Teffs, (d) CD4+FOXP3+, (e) CD4+FOXP3+HELIOS+, (f) CD4+CD31+, (g) CD4+CTLA+, (h) CD4+ItgB7+, (i) CD4+ItgA4B7+ over the course of treatment. Differences in means (± SD) for each dependent variable grouped by time on treatment were determined by one-way ANOVA and Dunnett's post hoc test where p ≤ 0.05 compared to baseline (*).