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. 2021 May 11;24(1):510. doi: 10.3892/mmr.2021.12149

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — GAS5 knockdown alleviates LPS-induced inflammation and apoptosis in THP-1 cells. (A) The knockdown efficiency of GAS5 expression was detected by RT-qPCR. The effect of GAS5 knockdown on the expression of inflammatory factors IL-6, TNF-α and IL-1β was analyzed by (B) RT-qPCR and (C) ELISA in LPS-induced THP-1 cells. The effect of GAS5 knockdown on apoptosis was determined by (D) flow cytometry and (E) the western blotting of apoptosis-associated proteins in LPS-induced THP-1 cells. *P<0.05, **P<0.01 and ***P<0.001 vs. control; ^##P<0.01 and ^###P<0.001 vs. LPS+sh-NC. GAS5, growth arrest-specific 5; LPS, lipopolysaccharide; RT-qPCR, reverse transcription-quantitative PCR; sh-, short hairpin; NC, negative control; PARP, poly (ADP ribose) polymerase.