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. 2021 May 6;12:641059. doi: 10.3389/fmicb.2021.641059

FIGURE 2.

FIGURE 2

R31 of NS1 is important for the electrostatic interaction between NS1 and negatively charged lipids. (A) NS1 R31E mutant can hardly bind to liposomes containing PI4P. Positively charged arginine (R31) of NS1 was mutated to negatively charged glutamic acid (E). A co-floating assay was performed to examine the association of ZIKV NS1 with the liposomes. (B) NS1 R31E mutant lost the ability to remodel the PI4P liposomes. Liposomes containing PI4P were incubated with wild-type NS1 or R31E mutant. The average diameter of liposomes was detected by DLS. R31E mutant can hardly change the diameter of liposomes. (C) R31 located in the groove between β-roll and greasy finger on membrane association face. The data are presented as the mean ± SEM. The p-values were obtained from a two-tailed t-test. **P < 0.01, ***P < 0.001.