FIGURE 5.

Sac1 overexpression interfered with NS1 induced ER remodeling and viral replication. (A,B) Sac1 overexpression interferes with NS1 induced ER remodeling. HeLa cells were co-transfected with Myc-NS1 and Flag-Sac1. Then cells were stained with anti-Myc and anti-Flag antibodies (A). Scale bar, 20 μm. The ratio of ER-remodeled cells to Myc and Flag double-positive cells is calculated as the percentage of ER-remodeled cells (B). (C) Sac1 overexpression impairs viral replication. BHK-21 cells were transfected with Sac1 expressing plasmid or control plasmid and then infected with ZIKV. The amount of viral RNA in the cell lysate at 48 hpi was quantified by RT-qPCR. The data are presented as the mean ± SEM. The p-values were obtained from a two-tailed t-test. ** P < 0.01, ***P < 0.001.