Skip to main content
. 2021 May 6;12:641059. doi: 10.3389/fmicb.2021.641059

FIGURE 6.

FIGURE 6

PIK inhibitors interfere with NS1 induced ER remodeling and viral replication. (A) PIK inhibitors decrease NS1 induced ER remodeling. NS1 expressed HeLa cells were treated with PIK inhibitors, wortmannin, or IN-9 at the indicated concentration. (B) Wortmannin or IN-9 inhibits ZIKV replication. BHK-21 cells were transfected by ZIKV replicon RNA. At 9 h post-transfection, cells were treated with indicated inhibitors. At 36 h post-transfection, luciferase activity was measured to evaluate viral replication. (C) The combination of Wortmannin and IN-9 shows improved inhibitory effects. ZIKV replicon RNA transfected BHK-21 cells were treated with Wortmannin and IN-9 at 9 h post-transfection. Luciferase activity was monitored at 36 h post-transfection. (D,E) Antiviral effects of PIK inhibitors. ZIKV infected BHK-21 cells were treated with Wortmannin (D) or IN-9 (E). Viral RNA in cell lysate was quantified by RT-qPCR at 48 hpi. The data are presented as the mean ± SEM. The p-values were obtained from a two-tailed t-test. *P < 0.05, **P < 0.01, ***P < 0.001.