Figure 2.

Simultaneously collected correlative AFM-confocal images of the human fungal pathogen C. albicans. Shown is the localization of tubulin2-GFP (green), histone protein B-RFP (purple) and reactive oxygen species (Cell-ROX-deep red, red) following exposure to the herbicide 2,4-D, which chemically induced oxidative stress. Tricolored confocal images are overlaid onto AFM images before and after the induction of ROS (A–D). Confocal images in the absence of 2,4-D (E–H) are compared with those 5 min after 2,4-D exposure (I–L) showing ROS induction (L). (E, I) are tricolor merged confocal images; (F, J) shows only RFP, G and K show only GFP and (H, L) show only ROS. The figure has been reproduced from Bhat et al. (2018b) with permission from Scientific Reports.