Fig. 1.

SENP3 is highly expressed in VSMCs following low shear stress-induced and Ang II-induced vascular remodeling. (a) Representative images of H&E staining and quantification of intima-media area and intima-media thickness in sham control and ligation of left common carotid artery (ligation) groups (*p < 0.05, n = 8). (b and c) Representative images of DHE staining and quantification of DHE staining intensity in control and ligation groups (*p < 0.05, n = 6). (d and e) Representative images of dual immunofluorescence staining of SENP3 and smooth muscle (SM)-α-actin and quantification of percentage of SENP3-positive VSMCs in control and ligation groups (*p < 0.05, n = 6). (f) Representative images of H&E staining, dual immunofluorescence staining of SENP3 and SM-α-actin, and quantification of percentage of SENP3-positive VSMCs in thoracic aortas from Ang II-infused mice compared with control mice (*p < 0.05, n = 6). (g) Western blot analysis of SENP3 protein expression in thoracic aortas from Ang II-infused mice compared with control mice. Values are mean ± SEM.