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. 2021 May 14;67:103386. doi: 10.1016/j.ebiom.2021.103386

Fig. 3.

Fig 3

SENP3 expression is upregulated by oxLDL and Ang II in a ROS-dependent manner in primary rat VSMC. (a) Primary rat VSMCs were treated with oxLDL (40 μg/ml) for the indicated time points and increasing concentrations of oxLDL for 30 min, respectively. Protein expression of SENP3 was examined by western blotting (n = 3, *p < 0.05). (b) Primary rat VSMCs were stimulated with Ang II (500 nM) for the indicated time and increasing concentrations of Ang II for 1 h, respectively. Protein expression of SENP3 was examined by western blotting (n = 3, *p < 0.05). (c) Primary rat VSMCs were treated with oxLDL (40 μg/ml), Ang II (500 nM) and H2O2 (100 μM) for 30 min. ROS level was detected by flow cytometry after DCFH-DA staining (n = 3, *p < 0.05 vs Con). (d and e) Primary rat VSMCs were stimulated with H2O2 (100 μM) for the indicated time (d) and increasing concentrations of H2O2 for 1 h (e), respectively. (f) Primary rat VSMCs were pretreated with or without 5 mM NAC for 6 h before Ang II stimulation for another 1 h. Protein expression of SENP3 was examined by western blotting (n = 3, *p < 0.05 vs Ang II/NAC(-), #p < 0.05 vs Ang II(+)). Values are mean ± SEM.