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Expression of MUC1 in mouse embryonic stem (mES) cells. The cells were differentiated with retinoic acid (RA, 10 μM) for the indicated period. (A) The relative expression levels of Oct4 and MUC1 mRNAs were analyzed by quantitative real-time PCR. Each bar is expressed as the mean±standard deviation (SD) of three experiments. ***p<0.001 (vs RA 0D). The protein level of MUC1-C was analyzed by western blotting (B) and flow cytometry (C).
