Fig. 4.

Inhibition of MUC1-C induces cell cycle changes and cell death in mES cells. (A) Cell cycle analysis of mES cells by flow cyto-metry. The cells were treated with the indicated concentration of CP1 or GO201 for 24 hr. (B) Apoptosis of mES cells treated with CP1 or GO201 for 24 hr was assessed by Annexin V/PI staining. Each bar is expressed as the mean±SD of three experiments. *p<0.05, **p<0.01, ***p<0.001 (vs CP1). The results at the bottom are shown as percentages of dead cell and apoptotic cell population. (C) Expression levels of Mcl1 and Bcl2 were analyzed by western blotting, after treatment with CP1 or GO201 at the indicated concentrations for 24 hr. Con, Control; CP, CP1; GO, GO201.