Figure 4.

General and distinct P-5-interacting protein annotation in cancer and immune cells

(A) (Left panel) P5-binding protein activity is mediated via membrane proteins, and the majority (55%) of membrane proteins in P5-interactome are transmembrane (dotted line). (Right panel) The P5-interactome acts through the nucleus, mitochondria, and ER.

(B) Compartmentalization of the 54 organellar proteins enriched from the two cell types.

(C) Annotation of the 62 protein high-confidence P5-interactome.

(D) Functional classification of P5-binding proteins from prostate cancer cells.

(E) Functional categorization of P5-binding proteins from CD8+ T cells.

(F) The main carnitine shuttle proteins that regulate β-oxidation are P5 targets. Color codes are cell-type-specific; (red) for both cell types and (blue) for only cancer cells.

(G) Many cholesterol biosynthesis enzymes are P5 targets. The enzymes from each step of the pathway are color coded (same as above), showing common and distinct cell-type-specific P5 targets.