Fig. 6.

Bone in-growth and osseointegration of the cisplatin/hydrogel-loaded 3D-printed Ti₆Al₄V implants in vivo. In the representative images of undecalcified histological sections stained with Masson Goldner's trichrome at 4 (A) and 8 (B) weeks after surgery, the mineralised bone tissues are stained green, the osteoid tissues are stained red/orange, and the implants appear black. The quantitative results of bone in-growth (C and E) and osseointegration (D and F) in the different groups were obtained using Image-Pro Plus 6.0 software based on two middle longitudinal sections. Data are represented as mean ± standard deviation (n = 10). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the implant group; #p < 0.05, ##p < 0.01, ###p < 0.001 compared with the implant + hydrogel + cisplatin 0.8 mg/mL group; &p < 0.05, &&p < 0.01, &&&p < 0.001 compared with the implant + hydrogel + cisplatin 1.6 mg/mL group. Scale bars = 200 μm.