Figure 1.

An inducible system for overexpression of Rav1 and Rav2-FLAG.A, scheme for overexpression of RAV1 and RAV2-FLAG. B, Western blot to analyze Rav1 expression before and after galactose induction. Cell lysates derived from equivalent numbers of cells from uninduced and induced diploids, as well as a rav1Δ strain, were subjected to SDS-PAGE and run on two separate gels. The top blot was probed with rabbit anti-Rav1 antibody, and the bottom blot was probed with mouse monoclonal antibodies against the A and B subunits of the V-ATPase. V-ATPase, vacuolar H+-ATPase.