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Chemical acetylation facilitates the analysis of histone methylation.A, histone H4 amino acid sequence. Commonly detected modifications are indicated. B, H4 (1–20) peptides containing methylation (me1 and me2) at R3 as reported by top-down MS analysis (14). Chemical acetylation collapses all the differently acetylated form into one. C, chemical acetylation collapses the 68 differently modified forms of H4 (1–20) detected in Phanstiel et al. (14) into six fully acetylated species.
