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Completeness of chemical acetylation and propionylation of histones in nuclear extracts determined by percent derivatization of various histone H3 and H4 tryptic peptides
| Peptide | Sequence | Max number of acetyl Lys | % Acetylation | % Propionylation |
|---|---|---|---|---|
| H4 (25–36) | DNIQGITKPAIR | 1 | 99.8 | 98.4 |
| H4 (80–93) | KTVTAMDVVYALKR | 2 | 99.5 | 99.9 |
| H4 (69–79) | DAVTYTEHAKR | 1 | 99.9 | 98.8 |
| H3 (74–84) | EIAQDFKTDLR | 1 | 99.7 | 96.9 |
