Table 2.
Confirmed H3 and H4 methylated peptides and methyl marks detected by bottom-up analysis of a chemically acetylated pool of nuclear extracts from Z-138, Toledo, HeLa, and MDA-MB-468 cancer cell lines
| Histone | Peptide | m/z | Charge | Peptide sequence | Methyl | Methylated residue(s) | Mascot score |
|---|---|---|---|---|---|---|---|
| H3 | 1–17 | 656.7093 | 2+ | ARTKQTARKSTGGKAPR | 2 | K4me2 | 41 |
| 9–17 | 500.2812 | 2+ | KSTGGKAPR | 1 | K9me1 | 52 | |
| 486.2839 | 2+ | KSTGGKAPR | 2 | K9me2 | 40 | ||
| 493.2941 | 2+ | KSTGGKAPR | 3 | K9me3 | 62 | ||
| 500.2812 | 2+ | KSTGGKAPR | 1 | K14me1 | 45 | ||
| 507.2901 | 2+ | KSTGGKAPR | 2 | K9me1K14me1 | 43 | ||
| 18–26 | 542.8272 | 2+ | KQLATKAAR | 1 | K18me1 | 41 | |
| 542.8272 | 2+ | KQLATKAAR | 1 | K23me1 | 48 | ||
| 73–83 | 696.3587 | 2+ | EIAQDFKTDLR | 1 | K79me1 | 88 | |
| 682.3641 | 2+ | EIAQDFKTDLR | 2 | K79me2 | 57 | ||
| H3.1 | 27–40 | 787.4420 | 2+ | KSAPATGGVKKPHR | 1 | K27me1 | 106 |
| 787.4420 | 2+ | KSAPATGGVKKPHR | 1 | K36me1 | 100 | ||
| 773.4452 | 2+ | KSAPATGGVKKPHR | 2 | K27me2 | 104 | ||
| 773.4452 | 2+ | KSAPATGGVKKPHR | 2 | K36me2 | 82 | ||
| 780.4518 | 2+ | KSAPATGGVKKPHR | 3 | K27me3 | 102 | ||
| 794.4489 | 2+ | KSAPATGGVKKPHR | 2 | K27me1K36me1 | 106 | ||
| 780.4518 | 2+ | KSAPATGGVKKPHR | 3 | K27me1K36me2 | 88 | ||
| 780.4518 | 2+ | KSAPATGGVKKPHR | 3 | K27me2K36me1 | 89 | ||
| 766.4553 | 2+ | KSAPATGGVKKPHR | 4 | K27me2K36me2 | 74 | ||
| 787.4616 | 2+ | KSAPATGGVKKPHR | 4 | K27me3K36me1 | 99 | ||
| 773.4645 | 2+ | KSAPATGGVKKPHR | 5 | K27me3K36me2 | 62 | ||
| H 3.3 | 27–40 | 795.4405 | 2+ | KSAPSTGGVKKPHR | 1 | K27me1 | 109 |
| 795.4405 | 2+ | KSAPSTGGVKKPHR | 1 | K36me1 | 109 | ||
| 781.4429 | 2+ | KSAPSTGGVKKPHR | 2 | K27me2 | 44 | ||
| 781.4429 | 2+ | KSAPSTGGVKKPHR | 2 | K36me2 | 76 | ||
| 788.4500 | 2+ | KSAPSTGGVKKPHR | 3 | K27me3 | 102 | ||
| 802.4475 | 2+ | KSAPSTGGVKKPHR | 2 | K27me1K36me1 | 109 | ||
| 788.4500 | 2+ | KSAPSTGGVKKPHR | 3 | K27me2K36me1 | 54 | ||
| 788.4500 | 2+ | KSAPSTGGVKKPHR | 3 | K27me1K36me2 | 77 | ||
| 774.4533 | 2+ | KSAPSTGGVKKPHR | 4 | K27me2K36me2 | 57 | ||
| 795.4579 | 2+ | KSAPSTGGVKKPHR | 4 | K27me3K36me | 89 | ||
| 781.4630 | 2+ | KSAPSTGGVKKPHR | 5 | K27me3K36me2 | 52 | ||
| H4 | 1–17 | 897.9998 | 2+ | SGRGKGGKGLGKGGAKR | 1 | R3me1 | 81 |
| 905.0074 | 2+ | SGRGKGGKGLGKGGAKR | 2 | R3me2 | 41 | ||
| 4–17 | 726.9166 | 2+ | GKGGKGLGKGGAKR | 1 | K5me1 | 67 | |
| 726.9166 | 2+ | GKGGKGLGKGGAKR | 1 | K8me1 | 46 | ||
| 726.9166 | 2+ | GKGGKGLGKGGAKR | 1 | K12me1 | 69 | ||
| 726.9166 | 2+ | GKGGKGLGKGGAKR | 1 | K16me1 | 51 | ||
| 20–35 | 640.7178 | 3+ | KVLRDNIQGITKPAIR | 1 | K20me1 | 51 | |
| 631.3854 | 3+ | KVLRDNIQGITKPAIR | 2 | K20me2 | 73 | ||
| 68–92 | 998.1979 | 3+ | DAVTYTEHAKRKTVTAMDVVYALKR | 3 | K77me3 | 61 |