Table 3.
N-terminal H4 peptides from Z-138 cell nuclear extract identified by data-dependent LC-MS/MS following derivatization with either acetic or propionic anhydride
| Peptide | Sequence | Acetyl | Methyl |
|---|---|---|---|
| In vitro acetylation | |||
| 1–17 | SGRGKGGKGLGKGGAKR | 5 | 1 |
| 1–17 | SGRGKGGKGLGKGGAKR | 5 | 2 |
| 4–17 | GKGGKGLGKGGAKR | 4 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 4 | 1 |
| In vitro propionylation | |||
| 1–17 | SGRGKGGKGLGKGGAKR | 4 | 0 |
| 1–17 | SGRGKGGKGLGKGGAKR | 3 | 0 |
| 1–17 | SGRGKGGKGLGKGGAKR | 3 | 1 |
| 1–17 | SGRGKGGKGLGKGGAKR | 2 | 0 |
| 1–17 | SGRGKGGKGLGKGGAKR | 2 | 1 |
| 1–17 | SGRGKGGKGLGKGGAKR | 1 | 0 |
| 1–17 | SGRGKGGKGLGKGGAKR | 1 | 1 |
| 1–17 | SGRGKGGKGLGKGGAKR | 1 | 2 |
| 1–17 | SGRGKGGKGLGKGGAKR | 0 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 4 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 3 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 2 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 1 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 0 | 0 |
| 4–17 | GKGGKGLGKGGAKR | 0 | 1 |
Precursors were sequenced by targeted PRM to determine the sites of modification reported in supplemental Table S2.