Figure 4.

HOPX and SOX2 contribute to the expression of Th17 cytokines

Expression levels of RORC (A), HOPX (B,F), and SOX2 (C,F) transcripts; IL-17A, IL-17F, IFN-γ, PDGF-AA, PDGF-AB/BB, LT-α proteins (D); and IL26, IL6, IL1A, IFNK transcripts (E), were analyzed by q-PCR (transcript) or ELISA and Luminex (protein) at 5 days of cell differentiation in Th17 cells (TGF-β, IL-6, IL-1β and IL-23) treated with RORC (siRORC; n = 4), HOPX (siHOPX; n = 8), SOX2 (siSOX2; n = 6) siRNA. Each set was compared with Th17 cells treated with non targeting siRNA (siNT) (n = 4 for RORC experiments, n = 8 for HOPX experiments, n = 6 for SOX2 experiments). Transcriptional results were normalized on RPL34 expression. All data are presented as fold versus siNT. Data are represented as mean ± SEM. (Student's T-tests; ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001).