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. 2021 Apr 30;24(5):102492. doi: 10.1016/j.isci.2021.102492

Figure 5.

Figure 5

HOPX and SOX2 contribute to the regulation of Th1/17 cell features

Expression of HOPX transcript (A) and IL-17A protein (B) in total CD4+ T cells treated with nontargeting (siNT) or HOPX (siHOPX) siRNA was analyzed by q-PCR and ELISA, respectively (n = 8). Expression of SOX2 transcript (C) and IL-17A protein (D) in total CD4+ T cells treated with nontargeting (siNT) or SOX2 (siSOX2) siRNA was analyzed by q-PCR and ELISA, respectively (n = 6). Frequencies of Th17 cells (CXCR3- CD161+ CCR6+) and Th1/17 cells (CXCR3+ CD161+ CCR6+) (E) were analyzed by flow cytometry in total CD4+ T cells treated with non targeting (siNT) (n = 8) or HOPX (siHOPX) siRNA (n = 8). Frequencies of Th17 cells (CXCR3- CD161+ CCR6+) and Th1/17 cells (CXCR3+ CD161+ CCR6+) (F) were analyzed by flow cytometry in total CD4+ T cells treated with non targeting (siNT) (n = 6) or SOX2 (siSOX2) siRNA (n = 6). Data are represented as mean ± SEM (Student's T-tests; ∗p ≤ 0.05; ∗∗∗p ≤ 0.001).