Figure 2.

Steps of liver dissection

(A) Midline incision of the skin.

(B) Midline incision of the muscle to expose organs.

(C) Ribcage cut on each side of the mouse. The white arrow and outline indicate the liver piece to be placed in the 2 mL tube. Alternatively, the liver piece can be taken from any desired lobe, or whole liver can be homogenized after freezing.

(D) Any generic dissection tools are suitable.

(E) Left, foil for freezing the rest of the liver for other assays. Right, 2-mL tube containing small liver piece for RNA extraction and sequencing.