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. 2021 May 13;2(2):100537. doi: 10.1016/j.xpro.2021.100537

Figure 2.

Figure 2

CITE-seq quality control

(A) Schematics of antibody staining process.

(B) Representative bioanalyzer electropherograms of CITE-seq antibody (ADT) library showing ADT product peak ~180 bp, which should be the predominant product peak. Smaller peak at ~140 bp is TSO-RT-Oligo product, which could be removed by further SPRI purification.

(C) Representative bioanalyzer electropherograms of RNA library with expected library size of ~300-700 bp.

(D) Representative CellRanger QC summary plot of mean reads per cell versus median genes per cell.

(E) Representative CellRanger QC summary plot of mean reads per cell versus sequencing saturation, with the far right data point indicating full sequencing depth of run.