CyTOF Antibody Volumes (per 50 μL Maxpar Cell Staining Buffer/sample)
| Component | Working dilution | Amount |
|---|---|---|
| anti-CD45-089Y (30-F11) | 1:100 | 0.5 μL |
| rat anti-CD25-151Eu (3C7) | 1:100 | 0.5 μL |
| anti-CTLA-4-154Sm (UC10-4B9) | 1:100 | 0.5 μL |
| anti-mouse PD-L1-153Eu (10F.9G2) | 1:100 | 0.5 μL |
| anti-mouse CD11c-142Nd (N418) | 1:100 | 0.5 μL |
| anti-mouse CD4-145Nd (RM4-5) | 1:100 | 0.5 μL |
| anti-mouse CD3e-152Sm (145-2C11) | 1:100 | 0.5 μL |
| anti-mouse PD-1-159Tb (29F.1A12) | 1:100 | 0.5 μL |
| CyTOF: rat anti-mouse ckit-166Er (2B8) | 1:100 | 0.5 μL |
| rat anti-mouse CD11b-148Nd (M1/70) | 1:100 | 0.5 μL |
| rat anti-mouse CD44-150Nd (IM7) | 1:50 | 1.0 μL |
| anti-NK1.1-165Ho (PK136) | 1:50 | 1.0 μL |
| CyTOF: anti-mouse CX3CR1-164Dy (SA011F11) | 1:50 | 1.0 μL |
| anti-CD86-172Yb (GL1) | 1:50 | 1.0 μL |
| anti-Ly-6C-162Dy (HK1.4) | 1:200 | 0.25 μL |
| rat anti-mouse Ly-6G-141Pr (1A8) | 1:200 | 0.25 μL |
| anti-I-A/I-E-209Bi (M5/114.15.2) | 1:200 | 0.25 μL |
| rat anti-mouse CD8a-168Er (53-6.7) | 1:200 | 0.25 μL |
| anti-mouse CD45R-144Nd (RA3-6B2) | 1:200 | 0.25 μL |
Make up fresh each use.