CITE-seq Antibody Volumes (per 50 μL CITE-seq Staining Buffer/sample)

Component	Working dilution	Amount
rat anti-CCR2/CD192 (SA203G11)	1:200	0.25 μL
rat anti-CD117/c-kit (2B8)	1:200	0.25 μL
rat anti-CD11b (M1/70)	1:200	0.25 μL
armenian hamster anti-CD11c (N418)	1:200	0.25 μL
rat anti-CD172a/SIRPα (P84)	1:200	0.25 μL
rat anti-CD25 (PC61)	1:200	0.25 μL
rat anti-CD3 (17A2)	1:200	0.25 μL
rat anti-CD38 (90)	1:200	0.25 μL
rat anti-CD4 (RM4-5)	1:200	0.25 μL
rat anti-CD44 (IM7)	1:200	0.25 μL
rat anti-CD45 (30-F11)	1:200	0.25 μL
rat anti-CD45R/B220 (RA3-6B2)	1:200	0.25 μL
rat anti-CD86 (GL-1)	1:200	0.25 μL
rat anti-CD8a (53-6.7)	1:200	0.25 μL
mouse anti-CD90.1 (OX-7)	1:200	0.25 μL
mouse anti-Cx3cr1 (SA011F11)	1:200	0.25 μL
rat anti-F4/80 (BM8)	1:200	0.25 μL
rat anti-I-A/I-E (M5/114.15.2)	1:200	0.25 μL
rat anti-Ly6C (HK1.4)	1:200	0.25 μL
rat anti-Ly6G (1A8)	1:200	0.25 μL
mouse anti-NK1.1 (PK136)	1:200	0.25 μL
rat anti-PD-1 (RMP1-30)	1:200	0.25 μL
rat anti-PD-L1 (MIH6)	1:200	0.25 μL
rat anti-CD169/Siglec-1 (3D6.112)	1:200	0.25 μL
rat anti-Siglec-H (551)	1:200	0.25 μL
mouse anti-TMEM119 (A16075D)	1:200	0.25 μL
mouse anti-XCR1 (Zet)	1:200	0.25 μL
rat anti-CD24 (M1/69)	1:200	0.25 μL
rat armenian hamster anti-CD103 (2e7)	1:200	0.25 μL
rat anti-CD49d (R1-2)	1:200	0.25 μL
rat anti-mouse Hashtag 1 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 2 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 3 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 4 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 5 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 6 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 7 antibody (M1/42; 30-F11)	1:200	0.30 μL
rat anti-mouse Hashtag 8 antibody (M1/42; 30-F11)	1:200	0.30 μL

Make up fresh each use.