Fig. 1.

Study design. Six to eight-week-old C57BL/6 GF male mice were placed on irradiated diet containing a mix of five fibers (assorted fiber diet; Af); a week later mice were colonized with one of two different human fecal samples SubA, SubB. Bedding and wires with food were exchanged between cages of mice colonized with the same community to minimize cage effects. Two weeks after colonization gnotobiotic mice received one of four isocaloric diets that vary by the type of fiber (10% w/w): cellulose (C), inulin (I), pectin (P), and assorted fiber (Af). Mice were maintained in these diets for 4 weeks