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. Author manuscript; available in PMC: 2022 Apr 9.
Published in final edited form as: ACS Infect Dis. 2021 Mar 10;7(4):894–905. doi: 10.1021/acsinfecdis.0c00917
Column:   Phenomenex Hyperclone C18 BDS 5 μm × 4.6 mm × 150 mm
Mobile phase:   A: water/acetonitrile 90/10, v/v, 0.15% TFA.
  B: acetonitrile/water 90/10, v/v, 0.15% TFA
Flow rate:   1 mL/min
Gradient:
Time (mins)       % mobile phase A
0          100%
20          40%
21          0%
23          0%
23.5          100
25          100
Detection:     210, 254 nm
Injection volume: 20 μL