Fig. 2.

PD1⁺ effector memory CD8⁺ T cells subsets and co-expression of inhibitory receptors in progressing and non-progressing CLL patients. a Absolute numbers of PD1⁺ EM and b PD1⁺ EMRA CD8⁺ T cells in progressing (left, n = 18) and non-progressing patients (middle, n = 10) at diagnosis and progression or non-progression. Fold change between time points of PD1⁺ EM and PD1⁺ EMRA CD8⁺ cells in progressing and non-progressing patients (right). c Representative viSNE plots of T cell differentiation subsets (naïve: CCR7⁺CD45RA⁺; central memory, CM: CCR7⁺CD45RA⁻; effector memory, EM: CCR7⁻CD45RA⁻ and EM CD45RA⁺, EMRA: CCR7⁻CD45RA⁺) and PD1 expression in CD4⁺ and CD8⁺ T cells at the two time points from one representative patient from each group. d Percentages of EM CD8⁺ cells co-expressing CD244 and PD1 (n = 12) and e CD160 and PD1 (n = 10). Graphs show mean ± SEM or paired values. (*P < 0.05; **P < 0.01; ***P < 0.001; Wilcoxon matched paired test or Mann-Whitney test)