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. 2021 May 21;19:110. doi: 10.1186/s12915-021-01045-w

Table 1.

Staging criteria for the specimens utilised in this study

Nomenclature HPF @ 20C Morphological criteria
8s 24h 8 somite embryos
1 GS 48h Larvae with a well-formed first primary gill slit
2.5 GS 60h Larvae with a well-formed first primary gill slit and the primordia of the second primary gill slit
5GS 3 months Larvae with 5 primary gill slits
9-10 GS 6 months Pre-metamorphic larvae with 10 primary gill slits**

HPF hours post-fertilisation

**B. lanceolatum larvae initiate metamorphosis when they have between 11 and 12 primary gill slits