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. 2021 May 21;21:94. doi: 10.1186/s12862-021-01823-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Genetic mapping of metabolism-related traits in Astyanax mexicanus. A Surface fish and Pachn cavefish were paired to produce F1 surface/Pachn hybrids. F2 hybrids were generated through interbreeding F1 hybrid siblings. B Hybrids were raised in group housing to 14months at which point they were housed individually in 1.5L tanks and fed 6mg of pellet food per day for 4months. Figure indicates the timeline for phenotype quantification. C Representative image of female F2 organs that were used to measure gastrointestinal tract length, visceral fat area (VAT), and liver area