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. 2021 May 21;49:41. doi: 10.1186/s41182-021-00330-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Comparison of MU VNTR profiles between the environment and humans. The relatedness between the genotypes was inferred using the UPGMA method in MEGA 7. Bootstrapping values for 1000 replicates are shown in percentage next to the branches. MIRU1 reference orthologs for M. marinum (the ancestor of MU), M. liflandii (another MPM), and M. ulcerans Agy99 (MU reference strain) were retrieved from GenBank with accession numbers given in the tree. MU: M. ulcerans; Sample test IDs and MU genotypes C, D, W are shown for environmental samples (B1NY, B6YE, W2YE, W7YE, W6AC) and BU lesions (H2ED, H3YE). The figure reveals clustering of MU genotypes from the environment and humans